HEXOKINASE

Report
HEXOKINASE
Korry Novitriani
20510045
Pendahuluan
Heksokinase EC. 2.7.1.1
 (2) menunjukkan nama kelas (transferase),
 (7 ) subkelas (fosfotransferase) dan
 (1) sub-sub kelas (fosfotransferase
dengan gugus hidroksil sebagai penerima),
 (1) D-glukosa sebagai penerima gugus
fosfat.

Hexokinase
Reaksi hexokinase telah ditemukan di
setiap organisme
 Glukosa
 ATP

Reaksi Heksokinase
Karakteristik Hexokinase
Ukuran : 50 kD - 100 kD
 pH Optimum : 7,5 - 9,0
 Titik isoelektrik: PI, 5,25 dan P-II, 4,93
 Stabilitas: suspensi kristal yang stabil
selama 6-12 bulan 2-8 ° C.
 Aktivator : Mg2+
 Inhibitor : Glukosa-6-Phospat

Jenis Heksokinase
 Heksokinase
I
 Heksokinase II
 Heksokinase III
 Heksokinase IV
Struktur Heksokinase
Preparasi Sampel
Isolasi Heksokinase
Sampel
Triton X-100
Percoll/ NaCl
NanoLC/ESI/MS/MS
Kemurnian
Daftar Pustaka


Toshihiko Aki and Shuichi Yanagisawa. “Application of Rice Nuclear
Proteome Analysis to the Identification of Evolutionarily Conserved
and Glucose-Responsive Nuclear Proteins”. Department of Applied
Biological Chemistry,The University of Tokyo, Japan, and Core Research
for Evolutional Science and Technology (CREST), Japan Science and
Technology Agency (JST), Kawaguchi, Saitama,Japan. 2009
Mioara Larion and Brian G. Miller. “Global Fit Analysis of Glucose
Binding Curves Reveals a Minimal Model for Kinetic Cooperativity
in Human Glucokinase”. Department of Chemistry and
Biochemistry, The Florida State University,Tallahassee, Florida. 2010

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