USE OF GOSSYPOL FREE COTTON SEED MEAL (Gossypium hirsutum) AS AN ALTERNATIVE PROTEIN SOURCE R. Navarro-Cortez,1 N. Flores,2 T. Wedegaertner, 3 E. Delgado.2 1Instituto 2 Tecnológico de Durango, Felipe Pescador 1830 Oriente, Nueva Vizcaya. CP: 34080. Durango, Dgo. México. New Mexico State University, Department of Extension Family and Consumer Sciences, MSC 3AE, P.O. Box 30003, Las Cruces, NM 88003. 3Cotton Introduction The United States is one of the main cotton producers in the world (Hilditch, 1948). Cottonseed meal (CSM) is a byproduct of the cotton oil industry, and is used for livestock feed or as fertilizer. CSM has a high protein content (42 to 50%), about 44 million metric tons of cotton seed are produced annually, which represent about 9.4 million metric tons of protein. This could provide the daily protein requirement for half a billion people (50 g/day) for one year, and help meet the requirements of half the population growth which is expected within the next 50 years (Sunilkumar et al., 2006; Wang et al., 2012). In addition, cotton is planted in regions with protein deficit such as Asia and Africa. New gossypol free cotton seed varieties from New Mexico State University can be use for protein production, and can be used as a substitute for soy protein. Incorporated, 6399 Weston Parkway Cary, NC 27513, USA Results Conclusions and implications The results show that water extraction and saline solution gave the lowest (p < 0.05) protein extraction and protein concentration, than KOH and combinations of water/KOH and NaCL/KOH at 55 and 5 ˚C, respectively. The highest protein content was extracted with 0.1N KOH solution at 55˚C. This condition also show the highest protein concentration (90%). Glandless cotton seed meal protein can be use as food additive in dairy, meat or cereal products to improve their nutritional and functional properties. Figure 1. Protein concentration in glandless cottonseed meal extracts at two different extraction temperatures. Objective The aim of this investigation is to develop a method to extract protein from gossypol free cotton seed meal. References Bush A. 1973. Low-Cost Protein from Cottonseed. Economic Botany, 27: 137-140. Hilditch T.P. 1948. Cotton seed: A pioneer crop and an example. Nature, 162:832-833 Sunilkumar G., Campbell LM., Puckhaber L., Stipanovic RD., and Rathore KS. 2006. Engineering cottonseed for use in human nutrition by tissue-specific reduction of toxic gossypol. Proceedings of the National Academy of Sciences, 103(48):18054-18059. Wang X, Tang J, Yao X, Wu Y, Sun H & Xu Y (2012). Effect of Bacillus cereus Br on bacterial community and gossypol content during fermentation in cottonseed meal. Afr. J. Microbiol. Res., 6: 6537-6544. Materials and Methods Figure 2. Protein purity of different glandless cottonseed meal extracts at two different extraction temperatures.