POPULATION SURVEY AND GENETIC CHARACTERIZATION OF MUTURU CATTLE IN NIGERIA A Research Proposal Submitted to the Livestock Production Research Committee, Institute of Food Security, Environmental Resources and Agricultural Research INTRODUCTION • Muturu: Bos taurus brachyceros • Trypanotolerant • Endangered: Dwindling population – decreased from 57,000 in 1977 to 38,000 in 1984 • • • • Adapted to sedentary farming Biodiversity enhancement through conservation Best bet for cattle production in Mandate States Study aimed at understanding population dynamics (Number, Herd Structure, etc.) Objectives of study • To undertake a detailed population survey of Muturu cattle breed in South and North Central regions of Nigeria. • To obtain information on some reproductive traits of economic importance. • To determine the incidence of trypanosome infection in the breed. • To undertake genetic characterization of the breed using blood protein variants. METHODOLOGY • • • • • Muturu population survey Haematological Analysis Prevalence of trypanosome infection Genomics studies Statistical analysis Table 1: Time-line Chart Main activity Phase 1 Population survey and collection of blood samples Sub activity - Location of existing Muturu Herd in the South-West and collection of blood samples - Location of existing Muturu Herd in the South-East and collection of blood samples - Location of existing Muturu Herd in the South-South and collection of blood samples - Location of existing Muturu Herd in the North Central and collection of blood samples Phase 11 Sample collation and processing - Population summary, Analysis of samples for haematological and biochemical parameters - Statistical analysis - Seminar/Conference Phase III DNA Analysis - Genotyping of blood samples Ma r Ap r Ma y Year 2010 Jun Jul Au Se g pt Oct No v De c Budget Item 1. Transportation/accommodation/feeding for 4 persons during field survey 2. 200 EDTA tubes/syringes and needles for collection of blood samples 3. Kits for analysis of haematological indices and biochemical parameters 4. 1 large/2 small coolers for field storage of samples 5. Ice block for storage of samples in the field 6. 3 litres of methylated spirit 7. 1 roll of cotton wool 8. 2 packs of hand gloves 9. Remunerations to farmers/Extension agents from ADPs as need may arise 10. GIS set 11. *DNA analysis Total Cost (N) 200,000.00 20,000.00 84,000.00 8,000.00 5,000.00 2,000.00 500.00 2,000.00 20,000.00 40,000.00 381,500.00 Project team S/N 1 Name Prof. O.A. Osinowo 2 3 Prof. O.M. Onagbesan Dr. I.J. James 4 Dr. M.N. Bemji 5 6 7 Dr. M.I. Takeet Mr. J. Oyedepo Mr. O. Omire 8 Dr. O. Ngozi Discipline Animal physiology Reproductive physiology Lactation Physiology Animal genetics & Breeding Parasitology GIS Postgraduate student Postgraduate student Phone No 08033187110 E-mail address email@example.com 08077766064 firstname.lastname@example.org 08060672833 email@example.com 08035397059 firstname.lastname@example.org 08037872682 08032485583 08076434738 07059211643 References Jabbar, M.A. and Diedhiou, M.L. 2001. Does breed matter to cattle farmers and buyers? Evidence from West Africa. FEEM Working Paper No. 110.2001. http://ssrn.com/abstract =297082. Raymond, M. and Rousset, F. 2001. Population genetics software and ecumenicism. J. Heredity 86, 248-249, http:/wbiomed.curtin.edu.au/genepop/. Rege, J.E.O., Aboagye, G.S. and Tawah, C.L. 1994a. Shorthorn cattle of West and Central Africa. I. Origin, distribution, classification and population statistics. FAO Corporate Document Repository – Identification and characterization of West African Shorthorn Cattle. World Animal Review, 78. Rege, J.E.O., Aboagye, G.S. and Tawah, C.L. 1994b. Shorthorn cattle of West and Central Africa. IV. Production characteristics. FAO Corporate Document Repository – Identification and characterization of West African Shorthorn Cattle. World Animal Review, 78.