Extraction of Nicotine By: DJ Hall and Brittany Hicks Purpose Determine if nicotine is extractable by means of supercritical fluid Run more than one sample type to determine if this makes a significant difference SFE Supercritical Fluid Extraction Uses high pressure CO2 Used to extract organics from different types of matrixes Allows for easy separation Problems All soluble organics will be separated form matrix GC-MS Gas Chromatography Mass Spectroscopy Separation of violate organics Determine the analyte separated at specific retention times using MS Nicotine (162.12g/mol) Poison (parasympathomimetic alkaloid) Formed in nightshade family of plants Antiherbivor chemical Small doses Stimulant Relaxation Large doses Fatal Extraction 2 Cigarettes Marlboro blend 27 2 Pieces Nicorette Gum 2mg 50 Drops of e-cigarette liquid 2.4% by weight Extraction cont. All extractions were obtained by bubbling into methanol and using SFE methanol modifier pump Cigarettes Ground using mortar and pestle Nicorette Gum Cut into small pieces E-cigarette liquid Celite as inert matrix Determination of Nicotine All samples were diluted to 50ml of methanol A standard was created by diluting 4mg of Nicotine in 50ml of methanol Standards and Samples were run using the GC-MS Reference Mass Spectra Standard Nicotine peak Cigarettes Nicotine peak Cigarettes cont. E-Cigarette Liquid Nicotine peak E-Cigarette Liquid Cont. Nicorette Gum Chromatogram Comparison E-Cigarette Liquid Nicorette Gum Cigarette Standard Conclusion It is possible to extract nicotine using the SFE Both the E-Cigarette and Cigarette samples contained nicotine The Nicorette Gum showed undetectable amounts of nicotine Nicotine may have be contained in the gum residue that was filtered before dilution Future Project Quantify the amounts of nicotine extracted Determine the extraction efficiency Work Cited Fischer, M. and Jefferies, T. Optimization of Nicotine Extraction from Tobacco Using Supercritical Fluid Technology with Dynamic Extraction Modeling. J.Agric. Food Chem. (1996), 44, 1258-1264.