Herpesviridiae Crista Wagner Herpesviridae Taxonomy Baltimore FAMILY I • 2XDNA mRNA directly • enveloped Greek herpein "to creep" • infection creeps latency acute • >130 genera • Varicellovirus: chicken pox & shingles • Cytomegalovirus: immunocompromised individuals • Simplexvirus: oral & genital herpes Simplexvirus Characteristics • human host, no animal reservoir • 60–95% of adults • transmitted via infected secretions • causes painful necrotic lesions • • herpes simplex encephalitis latent infection course • acute in epithelia, self-limiting ~14d • latent in neurons • recurrence triggered by various factors Simplexvirus Reproduction HSV Infection of the Neonate • 1/2000 infected during birth, 3/4 to asymptomatic mothers • Prevention: MOB acyclovir , caesarean delivery • SEM: 45% of cases, mortality rate 0% skin, eye and mouth lesions CNS or DIS • • • • CNS: 30% of cases, mortality rate 4% • central nervous system (encephalitis) • lethargy, fever, seizures, neurological dysfunction, brain destruction DIS: 25% of cases, mortality rate 29% • disseminated to multiple organ systems: liver & lungs • lethargy, fever, pneumonia, liver dysfunction, coagulopathy PCR for viral DNA, 14–21d IV acyclovir My Questions and Answers Question: Where are Simplexvirus during latent infection? Answer: sensory neurons No, no. Never, never do you want to have this slide as part of your presentation!!! Also…the answer is more detailed than what you said in your presentation! Herpesviridae Characteristics • Baltimore Group I • • • • • 2XDNA mRNA directly over 130 genera infect animals or humans Instead, try something subtle… enveloped latent infection course • • latent in neurons acute in epithelia Things to Remember • Dark background with white text is easier to see. • Background designs can be distracting. • More than ten words across the slide means your type is too small to be read. • Too many words on the slide means you’re being ignored and everyone is just reading. • Five minute presentations should have more than 7 slides. More than that, you’ve probably got too much information to finish on time.