Childhood Cancer and Blood Research (CCBR) BioBank, BC Children’s Hospital, Vancouver, BC The aim of the CCBR BioBank is to provide researchers with high quality biospecimens from pediatric patients with blood disorders in an ethical manner. The CCBR BioBank Mouse expansions The CCBR BioBank was established in September 2011 to collect and store biospecimens from pediatric patients with hematological disorders. The CCBR is governed by four committees; an oversight committee, a management and operations committee, a scientific committee and an advisory committee. The CCBR BioBank has the ability to expand our acute lymphoblastic leukemia cells in immune deficient mice allowing us to generate >100 times the number of primary leukemic cells for research purposes. This expansion provides sufficient numbers of highly enriched leukemia cells for the completion of multiparameter cellular and biochemical evaluations, including western blotting and flow cytometry experiments, as well as in vitro drug and growth factor response studies. Patients who donate their biospecimens to the CCBR BioBank understand that they are consenting for their biospecimens to be used in “as yet undetermined research projects”, they also know that their samples will be used in studies which have research ethics board (REB) approval and which have been reviewed by a committee of experts in association with the BioBank. Disease Representation in the CCBR BioBank Leukemias 38% 49% 12% How to apply for biospecimens from the CCBR BioBank All applications for biospecimens from the CCBR BioBank are reviewed by a Scientific Committee whose role is to assess the scientific merit of the application. The application procedure is outlined below. Apply to BioBank Coordinator by email to see if requested biospecimens are in CCBR BioBank inventory. Solid tumors with bone marrow involvement Solid tumors without bone marrow involvement Benign blood disorders 1% Fill out application form provided by BioBank Coordinator and attach: REB protocol REB application and certificate if obtained Proof of funding or application for funding PI CV Biospecimens The CCBR BioBank currently has bone marrow (primarily) biospecimens from pediatric patients with blood disorders including leukemia available for research purposes. In most cases blood and bone marrow specimens are ficoll separated to harvest viable polymorphic blood mononuclear cells (PBMC’s) which are stored in FCS + 10% DMSO in liquid nitrogen. We generate about 6 aliquots of ± 5 million cells from each patient sample. We also have permission to collect left over blood, DNA, CSF and stem cells from patients who participate in the CCBR BioBank For more information email: [email protected] Or visit www.cfri.ca/biobank Review by CCBR BioBank Scientific Committee and approval from CCBR BioBank Executive Committee for Management and Operations Signing of Material Transfer Agreement (MTA) and Release of biospecimens.