A Zebrafish Holding Chamber for Use with a Micro CT Scanner University of Wisconsin - Madison Biomedical Engineering Design Courses INTELLECTUAL PROPERTY STATEMENT All information provided by individuals or Design Project Groups during this or subsequent presentations is the property of the University and of the researchers presenting this information. In addition, any information provided herein may include results sponsored by and provided to a member company of the Biomedical Engineering Student Design Consortium (SDC). The above information may include intellectual property rights belonging to the University to which the SDC may have license rights. Anyone to whom this information is disclosed: 1) Agrees to use this information solely for purposes related to this review; 2) Agrees not to use this information for any other purpose unless given written approval in advance by the Project Group, the Client / SDC, and the Advisor. 3) Agrees to keep this information in confidence until the University and the relevant parties listed in Part (2) above have evaluated and secured any applicable intellectual property rights in this information. 4) Continued attendance at this presentation constitutes compliance with this agreement. Team Members Austin Ramme, Kristen Sipsma, Andrew Neumann, and Mike Haggerty Client Jill M. Kolesar, Pharm.D., BCPS Associate Professor of Pharmacy Advisor Professor Justin Williams Presentation Outline Problem Statement Background Information Current Devices Design Specifications Alternative Designs Final Design Future Work Problem Statement Client – An Evaluation of Carcinogenesis in a Zebrafish Model – Ultimate Goal: Cure Cancer Design Team – Design a Zebrafish Holding Chamber – Compatible with a Micro CT Scanner – Sustains Zebrafish Life Background Information CT = Computed Tomography Benefits of Zebrafish Research – Quick Breeders – Low Maintenance – Mapped Genome Zebrafish Anatomy – – – – Length: 1-6cm Lifespan: 2 years Water Temp: 64-75° F Bony Fish: Breathe Without Moving Typical CT Scanner Current Devices Market Products – Rodents – Zebrafish Rodent Tray Client’s Device – Cardboard – Zebrafish Slits – High Mortality Rate Client’s Current Device Preliminary Experimentation February 11, 2004 Conclusions – Survival: 1.5 mL for >30 min – Increased Breathing Rates – Preferred Orientation – No Adverse Behavior in Cuvette – No Adverse Behavior with Restraint Design Specifications No leakage Zebrafish must remain alive for 20 minutes Metal, glass, and dense materials must not be used Minimize all motion of the zebrafish Disposable or autoclavable Micro CT Scanner Design 1: Falcon Tube Falcon tube filled with water with sponge inside Small slits to hold fish One to two fish per tube Sponge absorption Design 2: Plexiglas Container Side View of Plate in Container Thumb – Screws 3D View of Container – No Plates In Place Multiple Fish Adequate Water Contamination Water 65mm 65mm Design 3: The Cuvette Cuvette Stabilization of Fish Alignment Tray Disposable/Inexpensive Water Supply Space Efficient Design Matrix Criteria: Cuvette Design Plexiglas Container Falcon Tube with Sponge Cost 4 3 3 Ease of Use 3 3 2 Constructability 5 3 4 Effectiveness: Immobilization 4 4 3 Effectiveness: Survival Rate 5 5 4 Totals 21 18 16 Each criterion was rated on a 0 to 5 scale with 5 being the best. Future Work Cuvette Tops – Rubber Stopper – Market Sizing Difficulties Opaque Materials Build Prototype Prototype Testing Questions ?