Feasibility Study of Pandan Leaves Extract

Report
and at AMERICAN MUSEUM OF NATURAL HISTORY,
New York, USA
April 21, 2014
Brief Description of the Equipment
• Knife
• Chopping Board
• Weighing Scale
• Measuring Glass
• Kettle
• Basin
• Hand Sprayer
• Stove
- used for slicing the leaves into smaller
pieces
- a board where leaves are sliced into
smaller pieces
- weighs the amount of leaves needed for
decoction
- measures the volume of water needed for
decoction
- container used for decoction of the
mixture
- used as container for the sliced pandan
leaves
- used as application tool
- supplies heat for decoction
General Procedure
Extraction
1. Researcher gather
pandan (Pandanus
odoratissimus L.) leaves
to be sliced into smaller
pieces using knife and
chopping board.
General Procedures
2. Weigh the amount of smaller pieces using the
weighing scale into the basin and put it in the kettle
with an amount of water using the measuring glass.
General Procedures
3. Heat the kettle with the use of
the stove for thirty minutes (30
minutes) for decoction.
General Procedures
Treatment 1
Partial picture of the
respondent using the
first treatment in trial
1 with 1 kilogram of
pandan leaves that
decocted in 1000 mL
of water.
Results and Interpretation of the Data
Table 1.1 Treatment A of Pandan extract to Dog No. 1
TREATMENT 1
(1 application of
treatment/day)
Decoction
PANDAN EXTRACT
USED IN TREATMENT
(mL)
NUMBER OF
REPELLED FLEAS
PANDAN
LEAVES
(Kg)
WATER
(mL)
Trial 1
1
1000
400
37
Trial 2
1
1000
400
25
Trial 3
1
1000
400
23
Trial 4
1
1000
400
19
Trial 5
1
1000
400
16
Results and Interpretation of the Data
Table 1.2 Treatment B of Pandan extract to Dog No. 2
TREATMENT 2
(1 application of
treatment/day)
Decoction
PANDAN EXTRACT
USED IN TREATMENT
(mL)
NUMBER OF
REPELLED FLEAS
PANDAN
LEAVES
(Kg)
WATER
(mL)
Trial 1
1
1500
400
34
Trial 2
1
1500
400
22
Trial 3
1
1500
400
19
Trial 4
1
1500
400
17
Trial 5
1
1500
400
15
Results and Interpretation of the Data
Table 1.3 Treatment C of Pandan extract to Dog No. 3
TREATMENT 3
(1 application of
treatment/day)
Decoction
PANDAN EXTRACT
USED IN TREATMENT
(mL)
NUMBER OF
REPELLED FLEAS
PANDAN
LEAVES
(Kg)
WATER
(mL)
Trial 1
1
2000
400
30
Trial 2
1
2000
400
20
Trial 3
1
2000
400
18
Trial 4
1
2000
400
13
Trial 5
1
2000
400
9
Results and Interpretation of the Data
• It shows that in every trial, there were decreasing
numbers of repelled fleas. The dog was treated once a
day in 5 consecutive days as represented by the number
of trials. There is an evident decrease on the number of
fleas on the dog after every treatment
Results and Interpretation of the Data
No. Of Fleas Repelled
28%
38%
Treatment 1
Treatment 2
Treatment 3
34%
Results and Interpretation of the Data
Conclusion
Recommendations
• That the pandan leaf extract be tested to a wide variety of
host animals that are also susceptible to dog fleas
(Ctenocephalides canis) like domestic cats and other
mammals.
Recommendations
• That in the depth study on the flea-repelling properties of
Pandan (Pandanus odoratissimus L.) be undertaken hence
the determination of the active repelling substance.
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