Series I Pump and Prep Pump Prior to removing the head, rotate the flag on the top of the motor so the piston is fully retracted. Remove thumb nuts or Allen nuts using a 3/16” Allen wrench and slide off the SST head. Remove inlet check valve holder with a 1/2” wrench and slide out SST check valve. Place SST check valve in new inlet holder noting the arrow on flow direction. Screw new inlet holder into the head and torque to 75 inch pounds. Remove one Phillips head screw from aluminum or SST spacer Hold pressure down on spacer while removing the second screw in order to keep from twisting and breaking the piston Slide out the spring and then the ceramic or SST piston Place small amount of grease on end of piston (May be SST or PEEK) and insert piston into the pump mechanism Slide spring back over the piston Reinstall the two Phillips head screws that secure the aluminum or SST spacer Slide the head over the piston and insert the two 5/32” Allen nuts or thumb nuts Working from side to side, lightly snug the nuts. Once seated, if Allen head nuts, torque the nuts to 30 to 35 inch pounds. Flow direction Outlet check valve and holder Flow Direction Inlet holder and check valve Note: arrows on check valves should be pointing up Mechanism Piston carrier Mechanism Piston Carrier roller bearing Front of pump Cam Piston Carrier Shaft Mechanism Flag Electronic Flag Sensor UHPLC Pump Prime/Purge Valve Prime/Purge Port Self Flush Pump Heads Flow Inlet “T” UHPLC Pump Outlet Filter Assembly Flow Check Valve Holders Single check valves torque to 130 in/lbs UHPLC Pump Outlet Filter Assembly UHPLC Pump Outlet Check Valve Holder Pump Heads Flow Inlet Check Valve Holder Procedure for Removing and Installing new seal Seal Removal and Insertion Tool Seal Insertion End Seal Removal End 5ml High Pressure Seal Seal Removal and Insertion Procedure Insert seal removal tool into head and remove SST or PEEK back up washer Seal Removal and Insertion Procedure Insert seal removal tool into head and press to one side and gently pull to remove seal Seal Removal and Insertion Procedure Clean seal port by inserting a corner of Scotch-Brite pad into hole and gently rotate. Rinse and use compressed air to remove any residue. Seal Removal and Insertion Procedure Place new seal on opposite end of extraction tool with SST spring exposed Seal Removal and Insertion Procedure Insert seal straight in making sure not to roll or pinch the seal’s edge Piston Replacement Manually rotate pulley until piston is in the retracted position. Having the piston in the retracted position decreases the chances the piston will break when removing the pump head. Retracted Front of Pump Not Retracted Head Removal and Piston Replacement Remove two Allen nuts or thumb nuts Slide head off Remove Self-flush housing Slide washer spacer off Head Removal and Piston Replacement Series II, III are clear or ceramic UHPLC piston is black Lift up piston from saddle Clean piston with Scotch-Brite. Rinse and use compressed air to remove any residue. Assemble parts in reverse order. Torque nuts to 30 in/lbs or hand tighten thumb nuts Head and Self-flush UHPLC Pump Pump Head and Self-flush Assembly UHPLC Pump High Pressure Head Assembly Outlet Inlet Single outlet Check valve Direction of flow Dual inlet Check valves UHPLC Pump High Pressure Check Valves Inlet of check valve has one hole Outlet of check valve has three holes UHPLC Pump Inlet and Outlet Check Valves UHPLC Pump Upper check valve holder Single Capsule torque to 120 in lbs Lower check valve holder Duel Capsule torque to 130 in lbs UHPLC Pump Inlet and Outlet Ports on High Pressure Head Outlet port has smaller (0.046) diameter hole Inlet port has larger (0.081) diameter hole Self Flush sits behind the high pressure pump head Flow UHPLC Pump Self Flush outlet Flow Self Flush inlet UHPLC Pump Self-flush Assembly Seal Direction of flow Outlet Check Valve Inlet Check Valve UHPLC Pump Self-flush seal replacement Seal tool Clean seal port by inserting a corner of Scotch-Brite pad into hole and gently rotate. Rinse and use compressed air to remove any residue. Seal Replacement Outlet If the backup washer is still in the seal cavity, insert the flanged end of the Teflon tool into the head and pull straight out. To remove the seal, reinsert the flanged end of the Teflon tool into the head, press to the side and pull straight out and remove the old seal. Gently clean the head with the Scotch-bright pad to remove any residue. Also, clean the piston with the pad to be sure the piston surface is clean. Do not use any other device other than the Teflon tool to remove and install the seal. Using a different device my scratch the head rendering it useless. To insert the new seal, position the seal on other end of the insertion tool so the stainless spring is positioned out and visible to the eye. Position the insertion tool so the seal will be pressed into the head without crimping, rolling or damaging the side of the seal. Press the seal into the head until it is firmly seated in the bottom of the seal port in the head. Reinstall the backup washer. Gently slide the head onto the pump. PEEK Head Assembly Inlet Outlet Outlet Assembly 1/16” Seat Port (2 Check Valve) Inlet Assembly 1/8” Seat Port (2 Check Valves) Direction of flow 1/8” Inlet Port 1/16” Outlet Port Torque holders to 15 in/lbs Mighty Mini Seal Replacement Prior to removing the head, rotate the flag on the top of the motor so the piston is fully retracted. Loosen two screws on the front of the head using 1/8” Allen wrench and slide head straight off Insert flared end of Teflon removal tool into center of seal, press to one side and pull out. Place new seal on opposite end of extraction tool with SST spring facing up. Press seal straight in. Do not insert seal in at an angle. Correct placement of seal PEEK spacer. Raised section should be inserted inward to hold seal in place. Incorrect placement of spacer. Raised section is facing out. Mighty Mini Check Valve Replacement Outlet Inlet Note arrows on Check valves Direction of flow Piston Replacement Remove two Phillips head screws Piston Replacement Remove spring Piston Replacement Remove Piston Piston Replacement SST Washer PEEK Spacer Spring Piston Piston Replacement PEEK Spacer Piston Spring SST Washer Piston Replacement Slide spacer with SST washer over the piston and retighten two Phillips Head screws. The small weep hole should be located at the bottom. Slide PEEK head back on and working from side to side, retighten the screws that secure the head. Old New Old New Old New Old New Old New Old New Old New Old New Old New Old New Old 110 Volt AC New 12 Volt DC Old New Old Cooling indicator New What is Viscosity? The measure of the resistance of a fluid to flow. What is Centipoise (cps)? Water is the standard by which all fluids are measured. Water is 1 cps @ 70 Degrees F. Water Milk SAE 10 Motor Oil SAE 20 Motor Oil SAE 30 Motor Oil SAE 40 Motor Oil Castrol Oil, Vegetable Oil Karo Syrup Honey Chocolate Syrup Ketchup Mustard 1 cps 3 cps 85-140 cps 140-420 cps 420-650 cps 650-900 cps 1,000 cps 5,000 cps 10,000 cps 25,000 cps 50,000 cps 70,000 cps Ways to help pump higher viscosity fluids: • Elevate the fluid • Increase temperature of the fluid • Pressurize vessel housing the fluid • Replace inlet and outlet check valves with larger diameter check valves Constant Flow: Typical pump configuration Most pumps are constant flow Pump delivers specified FLOW Does not take into account pressure If pressure readout – pulse dampener w/ transducer Max pressure limited to pressure of pulse dampener PEEK 5000 psi SST6000 psi Constant Pressure: Typical pump configuration Pump delivers specified PRESSURE Does not take into account flow……sometimes? No pulse dampener, uses Transducer “T” assembly Some pumps can be set above 9000 psi Pulse dampener w/ transducer (Constant Flow) PEEK Stainless Steel (SST) Transducer “T” Assembly (Constant Pressure) Constant Pressure: Does not take into account flow……sometimes? Constant pressure pump can be set up to run constant flow. 1) Press “Mode” button until Ml/Min LED is lit 2) Set flow rate to desired rate 3) Press “Mode” button until Hi/Pr LED is lit 4) Set hi pressure to a number that you know it will not reach running at the desired flow rate 5) Press Start/Run The pump will run up to the set flow rate and stay there.