Lipidomic

Report
Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION
Lipidomics
UCL
UCL
Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION
•
Same principle as the proteomic mass spectral method
•
Urine/plasma/CSF injected into a UPLC QTOF mass spectrometer
 lipids separated by UPC2 chromatography (supercritical fluid chromatography)
 Uses CO2 to separate lipids out according to their polar properties not non-polar (reverse phase)
 Lipids are easier to identify than metabolites as they have been more extensively studied
FFA Mix 1 FFA Method 0.5ul inj 15CV0
C22
17:14:13
TAGs_08022012_026
100
C20
C18
C16
C14
03-Aug-2012
1: TOF MS ESBPI
1.71e5
1.72
339.3196
1.52
311.2894
C24
1.35
283.2587
1.93
367.3502
1.18
255.2276
1.05
227.1970
%
Free fatty acids
C12
0.91
199.1659
C10
0.79
171.1348
C8
0
Time
0.20
0.40
0.60
0.80
1.00
1.20
1.40
1.60
1.80
2.00
2.20
2.40
2.60
2.80
Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION
•
Same principle as the proteomic mass spectral method
•
Urine/plasma/CSF injected into a UPLC QTOF mass spectrometer
UCL
 lipids separated by UPC2 chromatography (supercritical fluid chromatography)
 Uses CO2 to separate lipids out according to their polar properties not non-polar (reverse phase)
 Lipids are easier to identify than metabolites as they have been more extensively studied
Triacylglycerols
Chosteryl esters (
Mixture of FFA,
TAG, and CE
UCL
Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION
•
Phospholipid analyses from human heart
C34:0
8x107
Phosphatidylcholine
6x107
In te n s ity
C32:0
C36:0
C30:1
Phosphatidylethanolamine
4x107
Sphingomyelin
C36:1
ceramides
Phosphatidylcholine
Lyso-phosphatidylethanolamine
C36:3 Lyso
C38:3phosphatidyl
choline
2x107
Phosphatidylglycerol
0
0.50
1.00
1.50
2.00
2.50
Minutes
3.00
3.50
4.00
4.50

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