Slide 1

Report
Hairy Chinch Bug Biology and
Parasitoids in Ohio
Mrs. Jennifer Andon
Department of Entomology
The Ohio State University
Columbus, OH
2004-2006
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Objectives
Survey and document the annual populations
of hairy chinch bugs and their egg parasitoid in
Ohio lawn-type turf.
Determine if HCB populations obtained from
lawns being maintained at different levels of
management vary in susceptibility to Beauveria
bassiana infection.
Why?
Recent observations of hairy chinch bug
populations appears to conflict with earlier studies.
We have found HCB in irrigated lawns which were
thought to be at low risk for infestation.
The Culprit
Blissus leucopterus hirtus Montandon
Family: Blissidae
Formerly: Lygaeidae
Population Sampling Methods
• Standard golf course cup cutter
• U.S.A. standard sieve series
• Berlese funnel
EVALUATION OF BEAUVERIA
SUSCEPTIBILITY
Reared HCB colonies obtained
from irrigated and nonirrigated lawns on sorghum in
the greenhouse.
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Butyrate and
Plexiglas cylinders
Pro-mix
Peat Moss
16 hrs day light
75° F day temp
70° F night temp
Biology of Hairy Chinch Bug in Ohio
2004 & 2005 Data
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Two generations per year
Adults overwintered in lawns
Oviposition began in early May
1st generation nymphs peaked in mid-June
1st generation adults began egg lay by mid-July
2nd generation adults observed in early September
Reproductive diapause commenced in October,
chinch bugs descend into thatch and debris
Hairy Chinch Bug Populations in Ohio
Ex: Niemczyk & Shetlar 2000
Hairy Chinch Bug Populations 2004
600
Variable
TA /.099/6
TN /.099/6
TE /.099/6
500
# HCB/Sq Ft
400
300
200
100
0
April
May
June
July August Sept
Index
Oct
Nov
Hairy Chinch Bug Populations 2005
4000
Variable
Total Adults/sq. ft
Total Nymphs/sq ft
Total Eggs/sq ft
# HCB/Sq Ft
3000
2000
1000
0
April May
June
July
August Sept
Oct
Biological
Pathogens, Parasites, Predators
• Conserve Parasitoids and
Predators – Homeowner agreed not to
use insecticides during study.
Scelionidae
Eumicrosoma beneficum
Scelionid Egg Parasitoid
Scelionid adult
inside HCB egg
Parasitized and
normal egg
Other Natural Enemies
Bigeyed Bugs
Geocoridae
Predators
Beauveria bassiana
Hairy Chinch Bug cadaver with
B. bassiana.
Beauveria bassiana
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Deuteromycetes
“White Muscardine”
Ubiquitous, soil-borne fungus
Requires moist, warm
conditions to sporulate
Hyphae penetrate insect
cuticle
Toxin (beauvericin) relaeased
to kill insect
Mycelium proliferates inside
insect
Insect dies in 3-4 days
Beauveria bassiana Study
Except
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High maintenance lawns, i.e., regular
fertilization and irrigation, have been
observed with HCB infestations
Therefore, have populations of HCB
become less susceptible to Beauveria
infection?
I will evaluate Botanigard ES, a commercial
formulation of Beauveria bassiana and
determine if varying susceptibility to B.b.
has developed.
Beauveria, and
other microbes
from infected
cadaver
Isolated culture
of Beauveria
bassiana
Dilution Series
Botanigard™ ES Enumeration
10-1
10-2
10-3
10-4
16 billion CFUs per ml of
Botanigard ES
10 -5
10-6
What’s Next…
• Challenge different HCB populations
(recovered from different lawns) with
fungal spores.
• Repeat another field season of
monitoring chinch bug populations.
Acknowledgements – Thanks
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OSU/OARDC
OTF
Bayer, FMC, The Scotts Co.
Mark Grunkemeyer, Buckeye Ecocare
Scott Jones, One Call
Gary Chatriand, Laverlam Int’l
Columbus Area Master Gardeners
Dr. Dave Shetlar, Dan Digman
Dr. Mike Boehm, Amy Niver

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