4. STANDARD OPERATING PROCEDURES

Report
4.
STANDARD OPERATING
PROCEDURES
RYERSON UNIVERSITY
1
Biological Safety Training – Certificate Holder and User Training
BIOLOGICAL SAFETY
4. Standard Operating Procedures
Infection Control
Containment of Aerosols
Working in CL 1 areas
Working in CL 2 areas
Biological Safety
Cabinets
Human Blood and Body
Fluids
Needle Stick Injury
Prevention
Signs and Labels
Personal Protective
Equipment
Purchasing/Transfer
Inventory Control
Decontamination/Waste
Disposal
Large Scale Work
RYERSON UNIVERSITY
2
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Infection Control
Good microbiological practice:
is a basic code of practice to handle biological agents
Involves specific sterile techniques of product
protection, contamination control
- Danger
- Radiation
applied to all Rayonnement
types of work
involving
microorganisms
irrespective of containment level to reduce the risk of
exposure and infection
RYERSON UNIVERSITY
3
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Infection Control
Good microbiological practices prevent contamination of:
laboratory workers
the environment
experimental samples
Good work practices can significantly reduce the risk of:
Rayonnement - Danger - Radiation
aerosol production
contamination of experimental equipment surfaces
contains the biological agent
reduces the risk of infection
RYERSON UNIVERSITY
4
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Infection Control – Hand Washing
When to wash?
Before starting any manipulations
Before leaving the lab
When hands are obviously soiled
Before and after completing any task in a
biosafety cabinet
Rayonnement
- Danger
- Radiation
Every time
gloves are
removed
Before contact with one’s face or mouth
At the end of the day
RYERSON UNIVERSITY
5
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Infection Control – Hand Washing
Frequent hand washing has proven to be the
single most effective means of avoiding infection if
done properly and frequently
liquid dispensers should be used rather than bars
RYERSON UNIVERSITY
6
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Infection Control – Hand Washing
Wet hands with warm water
Dispense soap into a cupped hand
Spread around hands and between fingers
Wash hands for at least 10 sec.
Rinse thoroughly under warm water.
Dry hands thoroughly with paper towels.
RYERSON UNIVERSITY
7
Continue on to:
Containment of Aerosols
Return to SOP Main Menu
RYERSON UNIVERSITY
8
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Containment of Aerosols
Aerosols are gaseous suspensions of fine solid or liquid
particles ranging in sizes from 0.01 to 100 µm and can
remain suspended in air for extended periods of time.
Pathogens such as viruses and bacteria are so small
that they can travel within one aerosol droplet and be
dispersed by building ventilation.
Rayonnement - Danger - Radiation
Aerosols can settle on many surfaces where personnel
may unwittingly be exposed to a potentially infectious
material through the risk of direct contact.
RYERSON UNIVERSITY
9
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Containment of Aerosols
When appropriate, other primary barriers such as splash
shields, face protection or gowns should also be used.
When conducting procedures that create considerable
aerosols or when using agents classified at Containment
Level 2, work should be conducted in a Biological Safety
Rayonnement - Danger - Radiation
Cabinet
RYERSON UNIVERSITY
10
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Containment of Aerosols
The generation and dispersal of aerosols must be
minimized and controlled.
Numerous procedures and devices which can result in the
generation of aerosols:
pouring liquids,
using centrifuges, shakers, blenders,
Rayonnement -vessels,
Danger - Radiation
opening pressurized
inserting a hot loop into a culture,
pipetting, etc.
RYERSON UNIVERSITY
11
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Containment of Aerosols
Sterilization of inoculation loops in an open flame
may create aerosols which may contain viable
microorganisms.
Use a shielded electric incinerator.
Shorter handles minimize vibrations.
Disposable plastic loops are good alternatives.
RYERSON UNIVERSITY
12
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Containment of Aerosols
Mouth pipetting is prohibited.
All biohazardous materials should be pipetted in BSC’s.
Never force fluids out, use ‘to deliver’ pipettes.
To avoid splashes, allow discharge to run down
dispense the receiving container wall.
Never mix material
by suction
expulsion.
Rayonnement
- Dangerand
- Radiation
Reusable pipettes should be placed horizontally in a
disinfectant filled pan. Autoclave before reuse.
RYERSON UNIVERSITY
13
Continue on to:
Working in Containment
Level 1 & 2 Areas
Return to SOP Main Menu
RYERSON UNIVERSITY
14
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Working in CL 1 areas
The following requirements are basic for any laboratory
using biological agents. These requirements follow
Health Canada’s Laboratory Biosafety Guidelines 3rd ed
2004:
Good microbiological laboratory practices intended to avoid the
release of infectious agents are to be used.
A documentedRayonnement
procedural (safety)
must be available for all
- Dangermanual
- Radiation
staff and its requirements followed. The manual must be reviewed
and updated regularly
RYERSON UNIVERSITY
15
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Working in CL 1 areas
PROHIBITED:
• eating, drinking, smoking
• storing food or utensils
• applying cosmetics
• inserting or removing contact lenses
• oral pipetting
Rayonnement - Danger - Radiation
• loose long hair - must be tied back or restrained.
• doors to laboratories must not be left open (does not
apply to an open area within a laboratory)
RYERSON UNIVERSITY
16
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Working in CL 1 areas
WORK AREA
• laboratory must be kept neat, and clean
• storage of materials not pertinent to the work and cannot be easily
decontaminated must be minimized
• paperwork and report writing should be kept separate from
biohazardous work areas.
• access to laboratory and support areas is limited to authorized
personnel
• extreme caution Rayonnement
must be used- Danger
when handling
needles and syringes to
- Radiation
avoid autoinoculation and the generation of aerosols during use and
disposal. Needles must not be bent or sheared.
• disposable needles and syringes must not be replaced in their cap but
placed into a puncture-resistant container
RYERSON UNIVERSITY
17
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Working in CL 1 areas
WORK AREA
• technical procedures must be performed in a manner that minimizes
the creation of aerosols
• work surfaces must be cleaned and decontaminated with the
appropriate disinfectant at the end of the day and after any spill of
potentially hazardous material.
• loose or cracked work surfaces must be repaired or replaced.
• contaminated materials and equipment leaving the laboratory for
Rayonnement
Danger - Radiation
servicing or disposal
must be-appropriately
decontaminated and
labelled or tagged as contaminated
RYERSON UNIVERSITY
18
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Working in CL 1 areas
PERSONAL PROTECTIVE EQUIPMENT
• laboratory coats must be worn and fastened by all personnel
working in the laboratory.
• suitable footwear with closed toes and heels and preferably with
non-slip soles must be worn in laboratory
• protective laboratory clothing must not be worn in non-laboratory
areas;
• lab coats must not be stored in contact with street clothing
• suspected contaminated clothing must be decontaminated before
Rayonnement - Danger - Radiation
laundering
• eye protection must be worn when necessary to protect the eyes
• hands must be washed after gloves are removed, before leaving the
laboratory, and after handling materials known or suspected to be
contaminated, even when gloves have been worn
RYERSON UNIVERSITY
19
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Working in CL 1 areas
TRAINING:
Lab personnel must receive training on the potential hazards and the
necessary precautions to prevent exposure to infectious agents and
release of contained material
personnel must show evidence that they understood the training
provided
Rayonnement
- Danger
- Radiation
training must be
documented
and signed
by both the employee and
supervisor
retraining programs should also be implemented.
RYERSON UNIVERSITY
20
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Working in CL 1 areas
DISINFECTION & WASTE DISPOSAL
Disinfectants effective against the agents in use must be available at
all times within the areas where the biohazardous material is handled
or stored
effective concentrations and contact times must be used
all contaminate materials must be decontaminated before disposal or
reuse
efficacy monitoring of autoclaves used for decontamination with
biological indicators
must be
done regularly
Rayonnement
- Danger
- Radiation(i.e. consider weekly,
depending on the frequency of use of the autoclave)
records of these results and cycle logs (temperature, pressure) must
also be kept on file
RYERSON UNIVERSITY
21
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Working in CL 1 areas
ACCIDENTS
All spills, accidents (needlesticks, punctures, cuts, etc.) and overt or
potential exposures must be reported in writing to the laboratory
supervisor as soon as circumstances permit.
must file two reports:
Ryerson University’s Internal Accident Investigation Report filed
with the Centre for Environmental Health and Safety Management
(CEHSM) within 24 hours of incident
Workers’ Compensation Form (WSIB Form 7) form must be sent
Rayonnement - Danger - Radiation
to Human Resources
with 24 hours of incident.
Appropriate medical evaluation, surveillance, and treatment must be
sought and provided as required. Actions taken to prevent future
occurrences should be documented.
RYERSON UNIVERSITY
22
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Working in CL 2 areas
In addition to the safety precautions outlined for Containment Level 1
laboratory additional operational practices are outline below:
biological safety cabinets must be used for procedures that may
produce infectious aerosols and that involve high concentrations or
large volumes of biohazardous material.
Rayonnement - Danger - Radiation
appropriate door sign must be posted outside each laboratory
RYERSON UNIVERSITY
23
Continue on to:
Biological Safety Cabinets
Return to SOP Main Menu
RYERSON UNIVERSITY
24
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Biological Safety Cabinets
Biological Safety Cabinets (BSC) provide an effective means
of physical containment for biological agents, especially
when aerosols are generated.
The main role is to provide protection to personnel, and the
environment and product.
Rayonnement - Danger - Radiation
RYERSON UNIVERSITY
25
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Biological Safety Cabinets
Protection is achieved through the control of air
movement within and prior to leaving the cabinet, and
through the use of HEPA (high-efficiency particulate
air) filtration
Rayonnement
- Danger - Radiation
HEPA filters
are designed
to remove particles with
a minimum size of 0.3 microns with an efficiency of
99.97%.
RYERSON UNIVERSITY
26
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Laminar Flow Cabinets
Laminar flow cabinets (LFC) are similar in appearance, but
are not Biological Safety Cabinets:
•only protect the product
• intake room air which is passed through a pre-filter and a HEPA
filter to remove contaminants, dust and other particles
•purified air then enters the work surface in a laminar flow (nonturbulent) which is directed out of the cabinet or down into intakes
• these cabinets provide product protection only and must not be
Rayonnement - Danger - Radiation
used when working with any form of biohazard
•sometimes referred to as Clean Benches.
RYERSON UNIVERSITY
27
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Biological Safety Cabinets
Cabinet Certification
BSCs must be certified annually or after cabinet has
been moved
laminar flow hoods should be certified every second
year
 undertaken to ensure the HEPA filter has not be
damaged, leaking or plugged.
Rayonnement
- Danger - Radiation prior to any
 cabinet should
be decontaminated
certification activity.
HEPA filters & cabinets must be decontaminated prior to
disposal
RYERSON UNIVERSITY
28
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Biological Safety Cabinets
The effectiveness of a BSC is dependent upon:
the integrity of the cabinet – (the risk of exposure
increases if the integrity is jeopardized)
location of cabinet in a room
proper microbiological technique and work practices
that do not disturb established airflow velocity and cause
reverse currents
that can -re-introduce
contaminants into
Rayonnement
Danger - Radiation
the work area
continued maintenance and certification.
RYERSON UNIVERSITY
29
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Biological Safety Cabinets
Before using the cabinet:
Ensure BSC is certified
Disinfect work surfaces with
disinfectant
Place essential items inside
cabinet
Allow the blower to run for 5-10
min before work
RYERSON UNIVERSITY
30
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Biological Safety Cabinets
During use of a Biological Safety Cabinet:
ensure material and aerosol-generating
equipment is placed near the back of the hood
do not block any vents
use techniques that reduce splatter and aerosols
general work flow should be from clean to
contaminated areas
minimize movement so as not to impede air flow
RYERSON UNIVERSITY
31
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Biological Safety Cabinets
After completion of work:
Leave blower on at least 5
minutes to purge cabinet
Remove and decontaminate
equipment and materials
Disinfect cabinet surfaces
Turn off blower and fluorescent
lamp, turn on UV lamp
RYERSON UNIVERSITY
32
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Biological Safety Cabinets
Flames in Biological Safety Cabinets
The use of flame to sterilize equipment within a BSC is controversial. An
open flame in a BSC creates several major problems:
Flame creates turbulence, which disrupts the pattern of air supplied to
the work surface, therefore reducing maximum efficiency.
Heat build-up inside the BSC that may damage the HEPA filters
Creates a fire hazard.
Rayonnement
- Danger
Radiation
It is recommended
that flame
should-not
be used in BSC's.
Alternatives to flames include using disposable sterile inoculating
loops and needles, or pre autoclaved equipment in sterile packaging.
RYERSON UNIVERSITY
33
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Biological Safety Cabinets
Flames in Biological Safety Cabinets
If a flame is required, a touch-plate micro-burner equipped with a
pilot light to provide a flame on demand may be used.
This device will minimize internal cabinet air disturbances and
heat build-up.
During use, the heat source should be placed to the rear of the
workspace where resulting air turbulence will have minimal effect.
- Danger
An emergencyRayonnement
shut off valve
should- Radiation
be placed just outside the
BSC gas supply line and during the use of any burner, all
combustible materials and solvents must be removed.
RYERSON UNIVERSITY
34
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Biological Safety Cabinets
Ultraviolet Lamps:
Intended to destroy microorganisms in air or on exposed surfaces
Have limited penetrating power and only effective when properly
cleaned, maintained
Dust is attracted to the lamps which reduces the transmission of
the germicidal effect
Have a limited life span – even if the blue-violet glows, the lamps
are not effective if the terminal ends are blackened even slightly
Rayonnement
- Danger - hazards
Radiationsince UV light can be
UV lamps themselves
are potential
harmful to the eyes and skin and should therefore be turned off
when work is being conducted in the cabinet.
RYERSON UNIVERSITY
35
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Biological Safety Cabinets
Maintenance of a Cabinet:
Twice daily - Work surfaces wiped down
Weekly - UV lamp should be wiped clean
Monthly - All vertical surfaces wiped down
Annually - certification
RYERSON UNIVERSITY
36
Continue on to:
Human Blood and Body Fluids
Return to SOP Main Menu
RYERSON UNIVERSITY
37
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Human Blood and Body Fluids
blood borne pathogens, which are microorganisms that are
present in blood and bodily fluids and are capable of causing
disease in exposed individuals
exposure to human blood and bodily fluids (e.g., semen,
cerebrospinal, amniotic,), tissue cultures, or organ cultures
increases the risk
pathogens of greatest concern are hepatitis B virus, hepatitis C
virus and the Human Immunodefiency Virus (HIV)
Rayonnement - Danger - Radiation
RYERSON UNIVERSITY
38
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Human Blood and Body Fluids
RISK OF EXPOSURE DEPENDENT ON:
pathogen involved
type of body fluid
route of exposure
duration of exposure
volume of blood involved in exposure
concentration of virus at time of exposure
PPE worn
RYERSON UNIVERSITY
39
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Human Blood and Body Fluids
UNIVERSAL PRECAUTIONS:
The minimum standard of practice to prevent exposure to blood
borne pathogens and includes:
•
•
•
•
education
personal protective equipment
hand washing
safe work practices
Universal precautions assumes the material to be infectious and
Rayonnement - Danger - Radiation
required to use control measures to prevent skin and mucous
membrane exposure.
These precautions must always be used when handling blood or
body fluids.
RYERSON UNIVERSITY
40
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Universal Precautions
1. Gloves should be worn when handling potentially contaminated
surfaces.
2. Avoid touching items that are NOT contaminated when gloves are
being worn.
3. PPE should be worn during procedures that are likely to generate
droplets of blood or bodily fluids.
4. Contaminated lab coats and gloves should be removed immediately
after procedureRayonnement
is completed.- Danger - Radiation
5. Hand washing is the most important preventative tool - hands should
be washed immediately after gloves are removed and before leaving a
work area.
RYERSON UNIVERSITY
41
Continue on to:
Needle Stick Injury Prevention
Return to SOP Main Menu
RYERSON UNIVERSITY
42
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Needle Stick Injury Prevention
Injuries from sharps (needles, syringes, etc.)
may result from:
• lack of training on proper work practices
• crowded work conditions
• incorrect recapping of non retracting
needles
• poor disposal
practices
Rayonnement - Danger - Radiation
[risk of injury exists not only to the user, but also
support staff such as caretaking or hazardous waste
disposal personnel]
RYERSON UNIVERSITY
43
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Needle Stick Injury Prevention
Should a needle stick injury occur it is important
to report it immediately and seek a medical
assessment.
Such injuries must not be ignored - potential
Rayonnement - Danger
- Radiationcould develop
laboratory associated
infections
without appropriate treatment.
RYERSON UNIVERSITY
44
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Needle Stick Injury Prevention
To avoid needle stick injury use:
•
•
•
•
•
needle-less techniques where possible
retractable sharps
fill syringes carefully
do not bend, or recap needles
Rayonnementsharps
- Dangercontainers
- Radiation
approved designated
should be
used for disposal that will not allow penetration or
direct access to sharps
RYERSON UNIVERSITY
45
Continue on to:
Signs and Labels
Return to SOP Main Menu
RYERSON UNIVERSITY
46
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Signs & Labels
Biohazardous or infectious materials fall under;
Class D, division 3 of WHMIS
(Poisonous and Infectious
Material - Biohazardous Infectious
Material)
Class 6.2 of Transport Canada
Transport of Dangerous Goods
Act (Infectious Substances)
RYERSON UNIVERSITY
47
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Signs & Labels
ENTRANCES
Biohazard signs must be posted on doors to rooms
where biohazardous materials are used (Level 2 or
greater)
Rayonnement - Danger - Radiation
RYERSON UNIVERSITY
48
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Signs & Labels
Biohazard labels must also be placed on containers, equipment and storage units
(e.g., fridges, freezers) used for biological agents.
RYERSON UNIVERSITY
49
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Signs & Labels
INSIDE LAB
(posted
prominently)
Internal Ryerson Biosafety Certificate
Current list of authorized users
RYERSON UNIVERSITY
50
Continue on to:
Personal Protective Equipment
Return to SOP Main Menu
RYERSON UNIVERSITY
51
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Personal Protective Equipment
•
•
•
•
Lab coat (buttoned)
Disposable gloves
Safety glasses
Footwear
Responsibility of both the user and the supervisor to
ensure that PPE is appropriately worn
RYERSON UNIVERSITY
52
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Personal Protective Equipment
Personal protective equipment (PPE) is only effective if
correctly selected, fitted, used and cared for, and the
individual is trained
Criteria for consideration
• routes of exposure that need to be blocked
• degree of protection offered
• specific to each level of containment
Ensure PPE is removed before leaving the lab.
RYERSON UNIVERSITY
53
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Personal Protective Equipment
Lab Coats/Gowns
long-sleeved, knee length
buttoned or ideally with snaps
periodic cleaning required
lab coat should be worn in lab area only
RYERSON UNIVERSITY
54
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Personal Protective Equipment
Gloves
nitrile & vinyl for work with biological agents
avoid latex gloves if possible
consider tensile characteristics, length of cuff
remove gloves when walking in hallways
double glove or do not touch uncontaminated
items in lab when wearing gloves
RYERSON UNIVERSITY
55
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Personal Protective Equipment
Safety Glasses
safety glasses or goggles to protect the eyes
RYERSON UNIVERSITY
56
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Personal Protective Equipment
Footwear
closed toed shoes should always be worn
sandals or open toed shoes are not allowed in a
biohazard lab
RYERSON UNIVERSITY
57
Continue on to:
Purchasing/Transfer
Return to SOP Main Menu
RYERSON UNIVERSITY
58
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Purchasing
ALL PURCHASES OF CL 2 BIO AGENTS MUST BE
APPROVED BY BSO PRIOR TO PURCHASE
Only Certificate Holders or designated authorized users
may purchase biological agents
Import permits required from United States for certain animal
or human pathogens
Records of all purchases must be maintained for inspection
RYERSON UNIVERSITY
59
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Transfer
WITHIN UNIVERSITY
prior BSO approval to ensure appropriate containment
available
unauthorized lending or borrowing of biohazards not
permitted between labs
Rayonnement - Danger - Radiation
RYERSON UNIVERSITY
60
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Transfer of Human Cells/Tissue
OUTSIDE UNIVERSITY
prior BSO approval for lending or borrowing of biohazards from other
institutions before arrival on campus
valid Research Ethics Board (REB) approval is required prior to the transfer of
material
receiving institution must have appropriate containment
materials must be properly packaged and documentation
inventory must be adjusted
if material is being received by Ryerson, then a copy of the other
institution’s Research Ethic Board approval is also required
Online applications for Research Ethic Board approval is available at:
http://www.ryerson.ca/about/vpresearch/ethics.html
RYERSON UNIVERSITY
61
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Inventory Control
Certificate Holder required to use Inventory control
Form for all biological agents
Inventory records must be kept up to date and available
to the BSO for inspection
Types of biological organisms should be kept current
with the list noted in the Biosafety Certificate
Rayonnement - Danger - Radiation
Changes require a revision to the Biosafety Certificate
RYERSON UNIVERSITY
62
Continue on to:
Decontamination & Waste
Disposal
Return to SOP Main Menu
RYERSON UNIVERSITY
63
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Decontamination
There is no universal decontamination method
for biological materials
A spectrum of chemical and physical methods
for decontamination exist
Environmental and other factors can influence
efficacy
There is a specific vocabulary for
decontamination
RYERSON UNIVERSITY
64
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Decontamination Vocabulary
• Decontamination
• Disinfection or sterilization of contaminated surfaces
and/or articles to make them suitable for use
• Sterilization
• Act or process (physical or chemical) that destroys or
eliminates all forms of life, especially microorganisms
• Disinfectant
• An agent, usually chemical, that inactivates viruses or
kills vegetative microbes but not necessarily resistant
forms such as spores
RYERSON UNIVERSITY
65
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Decontamination
laboratory bench tops, other surfaces, and all
equipment contaminated with biohazards must be
decontaminated prior to reuse, servicing, transfer or
disposal.
procedures must be in place to ensure the effectiveness
of the methods used to decontaminate items.
RYERSON UNIVERSITY
66
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Chemical Decontamination
Choice depends on:
• Type of material to be disinfected
• Organic load
• Chemical characteristics
Most common are chlorine
compounds and alcohols (broad
range)
RYERSON UNIVERSITY
67
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Chemical Decontamination
•Chemicals include:
2% -10% domestic bleach (hypochorite solution)
70% Ethanol
Quaternary ammonia
6% formulated Hydrogen peroxide
10% formalin
2% glutaraldeyhye
phenolic compounds
RYERSON UNIVERSITY
68
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Physical Decontamination
Heat:
• Autoclaving (most practical and recommended)
• Incineration (for disposal of sharps and tissues)
Irradiation:
• UV light (wavelength of 253 nm is germicidal)
• Gamma (disrupts DNA and RNA)
Filtration
• HEPA (biological safety cabinets, ventilation)
• 0.2 micron (physically removes particulates )
RYERSON UNIVERSITY
69
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Sterilization by Autoclaves
sterilization is a process which
results in the total destruction of all
living and viable organisms (with a
probability of 1 in 1 million that 1
organism survived)
sterilization is achieved through
autoclaves
autoclaves utilize both pressure and
high temperatures to provide an
effective way of sterilizing items
RYERSON UNIVERSITY
70
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Waste Disposal
Ryerson is moving from autoclaving its
waste on-site to packaging waste for an
external company to pick up.
Such companies collect properly
packaged biological waste for industrial,
off-site autoclaving and/or incineration
RYERSON UNIVERSITY
71
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Initial Transition Phase
If you currently have filled autoclave bags
1. Set up on of the supplied cardboard boxes with
yellow plastic liner and place your bag inside (you
can put multiple bags until the yellow liner/box is full)
2. Tie up liner, close and tape shut the box
3. If your waste contains cytotoxic drugs (.e., chemo
agents), put a “cytotoxic” label on the box
4. With a marker, write lab name and date on side of
box (e.g., Smith lab/ Jan 12, 2015)
RYERSON UNIVERSITY
72
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Initial Transition Phase
RYERSON UNIVERSITY
73
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Waste Disposal Supplies
Colour Coding
– Yellow for regular biological wastes
– Red for cytotoxic wastes
Any material that may have come into contact with a
cytotoxic drug (such as chemotherapeutic agents) which
inhibit or prevent the function of cells.
RYERSON UNIVERSITY
74
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Waste Disposal Supplies
Pails
– Use pails to collect waste that could puncture through plastic
bags, such as serological pipettes, pipette tips, glass tubes,
slides, etc.
– Use pails to collect liquids – Do NOT pour liquids directly into
pails, keep liquids in small bottles, then tighten their lids and
place in pails
RYERSON UNIVERSITY
75
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Waste Disposal Supplies
Boxes with yellow liners
– For solid waste that cannot puncture a plastic bag
(e.g., contaminated gloves, paper towels,
unbreakable plastic bottles, etc.)
– For filled sharps containers
If cytotoxic,
add sticker
to box
RYERSON UNIVERSITY
76
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Waste Disposal Supplies
Each department will have a location where you
can pick up these supplies
– Current locations for
Chemistry & Biology department – KHN 202C
– Call main department to coordinate
– Other departments will need to find a common location
for supplies, for now, submit a request to
[email protected]
You can also email [email protected]
for more supplies if your department has run out
RYERSON UNIVERSITY
77
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Waste Disposal Process
When boxes are full, tie up
liner and tape box closed
When pails are full, close
lid tightly until it clicks
(locked – cannot be
opened again)
Label side of boxes and
white lid of pails with Lab
name and current date
– Example: Smith lab / Jan 12,
2015
RYERSON UNIVERSITY
78
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Waste Disposal Process
As of January 2015, biological waste pick-up will take place
every Tuesday
– Stay current by checking the website regularly:
http://www.ryerson.ca/content/ryerson/irm/programs_policies/bio_s
afety/waste-disposal.html
Each department will have a location where biological
waste can be dropped off every Monday afternoon
– Current locations for
Chemistry & Biology department – KHN 202D
– Call main department to coordinate
– Other departments will need to find a common location for waste
drop-off. For now, email [email protected]
RYERSON UNIVERSITY
79
For more information on changes to
biological waste disposal
• please see the Biosafety Program page
(http://www.ryerson.ca/content/ryerson/irm/programs_policies/bio_safety.html)
and
• click on the “updated waste procedures” link
(http://www.ryerson.ca/content/ryerson/irm/programs_policies/bio_safety/wast
e-disposal.html)
RYERSON UNIVERSITY
80
Continue on to:
Large Scale Work
Return to SOP Main Menu
RYERSON UNIVERSITY
81
Biological Safety Training – Certificate Holder and User Training
4. Standard Operating Procedures
Large Scale or High Concentrations
Containment levels based on laboratory scale operations:
increase in containment may be required if working
with high concentrations or shifting to manipulations
involving >10 litres
increase in the amount of potentially infectious
material may require additional safety precautions
and/or increase in containment
risk assessment should determine whether there is an
increase in aerosol generation
RYERSON UNIVERSITY
82

similar documents