Blood sample
EHES Training
• Centrifuge
• Empty tubes for recentrifugation (only
needed if gel serum
tubes are used)
• Storage tubes
• Pipette
• Storage boxes
• Disposable gloves
Before centrifugation of
blood tubes
• After blood drawing
store the tubes at room
temperature in a vertical
position at least 30
minutes for the blood in
serum tubes to be
• Do not prolong the
waiting time over 60
• Adjust centrifuge speed
2000-2200 g RCF
Centrifugation of blood
tubes (1/2)
• After the waiting
period, centrifuge
• The serum tube
• The fluoride-citrate tube
• The 1st EDTA tube
• Do not centrifuge
the last two EDTA
tubes designated for
HbA1c and DNA
Centrifugation of blood
tubes (2/2)
• Place the serum tube and
the fluoride-citrate and the
1st EDTA plasma tube in the
• Check that all tubes are
resting on the bottom of the
centrifuge rack
• Check that the centrifuge
rotor and tubes are in
• Centrifuge the tubes 2000 g
for 10 minutes at room
Sample handling after
• After the centrifugation,
check that the serum is
separated properly
• Pour the serum from
the 2 serum collection
tubes into the pooling
tube, mix gently by
swirling the contents
• Note! If one of the serum
tubes is haemolysed, do
not mix them together
Labelling storage tubes
• Place the storage tubes
in a tube rack according
to the pipeting scheme
• No need to label all
tubes if blood collection
is incomplete
• Place the bar code labels
in an upright direction,
so that the scale marks
remain visible
Handling blood collection
tubes with gel
• The temperature during
centrifugation should be
at least 20-22 °C
• Inspect the gel tube after
centrifugation for:
• Gel surface horizontal
• Serum and cell layers
separated clearly
• No red cells visible on top
of the gel
• No fibrin strands in the
• Serum liquid, not clotted
Hemolysed samples
• Do not pool a hemolysed
serum sample with nonhemolysed samples
• Several hemolysed
samples can be pooled
• Document hemolysed
• Use the colour guide to
document the degree of
Pipette aliquots into
storage tubes
• 2 x 1,5 ml serum from the
pooling serum tube into
the 3 ml plastic tube and
1,5 ml aliquots into the 1,5
ml storage cryotubes
• 2 x 1 ml from fluoridecitrate plasma tube into
two 1,5 ml cryotubes
• 2 x 1,5 ml from the EDTA
tube into two 1,5 ml
• Close the cryotubes with
caps and freeze the tubes
at once
Transferring storage
tubes into freezers
• Place the tubes
without delay into the
boxes in the freezer
• Label boxes before
placing them into the
freezer because labels
will not stick on a
wet/cold surface
Sample shipment
• To the national HES laboratory
• In dry ice
• Packed in leak proof secondary packaging
• To the EHES Reference Laboratory
• Packed according to the IATA regulations,
according to Pecking Instruction 650 for
diagnostic specimens
• Place one tube upside down in every box
• The amount of the collected
serum and plasma
• Visible attributes (haemolysis,
lipemia, icterius)
• Deviations from the sample
handling protocols, if any
• Your initials/personnel code
to identify who handled the
• Slides
• Laura Lund, Päivikki Koponen
• Photographs
• Hanna Tolonen
• Demonstration
• Saara Vallivaara

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