Houston 5977A GCMS Intro

Report
GC-MSD
Introducing the new 7890B/5977A
Badr Astiphan
GCMS Product Sales Specialist
1-866-524-7936
302-290-5631 cell
[email protected]
1
Agilent Confidential
January 8, 2013
We are First in GC
Continuously innovating
7000 GC
Triple Quad
Units Installed
>250,000 GC
>60,000 MS
7200 GC QTOF
Low Thermal Mass
Technology
Multi-Mode
Inlet
Retention Time
Locking
2nd Gen Linear
Electron Capture
Detector
Electronic Pneumatic
Control
6890 GC
Capillary Flow
Technology
Inert MS Ion
Source
Hyperbolic
Gold Quad
5970 MSD
Fused Silica
Capillary Column
5890 GC
AutoInjection
5840 GC
Microprocessor
Control
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5880 GC
Agilent Confidential
January 8, 2013
7890A/5975C GC/MSD:
The Most Popular GC/MS in the World
The industry standard
GCMS
Over 20,000
5975 systems since 2005
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January 8, 2013
Agilent 5977 MSD
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Agilent Confidential
January 8, 2013
Proven Technologies
Improving the Industry Standards for GC/SQ
Gold Quartz Quad
Triple Axis Detector
High Temperature
Inert Source
Unique Entrance
Lens Design
All of 5975’s features also built in 5977, such as “Gold Quad”, SIM/Scan, Gain
tuning, Triple-Axis Detector, Trace Ion Detection, Auto CI, etc..
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Agilent Confidential
January 8, 2013
High Temperature Ion Source
350°C
325°C
Solid inert material – not a coating
– Not damaged by multiple cleanings
Programmable to 350ºC
Improved response for many compounds
– More robust operation for “dirty” samples
– Better response for “sticky” compounds
– Sharper peaks for high boilers (late eluters)
300°C
275°C
230°C
Polyaromatic Hydrocarbon (PAH)
… more performance for a wide range of applications
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January 8, 2013
Unique Entrance Lens Eliminates Pre-filter
New, improved lens
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Agilent Confidential
January 8, 2013
Unique Entrance Lens Eliminates Pre-filter
Fringe fields
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January 8, 2013
Quartz – Gold – Hyperbolic and Beautiful!
Why quartz?
Much more than just a beautiful piece of glass
– Low coefficient of linear expansion
(expansivity; α)
• dL = α L0 dt [t = ºC; dL = change in
length (m); L0 = length at 0 ºC]
Why gold?
– Inert material with excellent electrical
properties for high voltage
Why hyperbolic?
– Ideal electrical field is generated by
hyperbolic surfaces (NOT ROUND)
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Round Quad
Agilent Confidential
January 8, 2013
Triple-Axis Detector
With a combination of three axes and appropriate
shielding, noise from secondary particles can be
reduced to a minimum
Offset
Z
Y
ion beam
X
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Agilent Confidential
January 8, 2013
Triple Channel EM
Exit of the triple channels
Triple channels improve signal
amplification
– Lower initial EM voltage required
Triple channels increase life
– Lower cost of operation
Collector removed to
show exit passages
Collector
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Agilent Confidential
January 8, 2013
Expanded Capabilities: 7890B/5977A
Improving the Industry Standards for GC/SQ
Save Time & Energy
Higher Ion Count
•Sequence Scheduler
• Extractor EI source
• Increased ion count
• S/N > 1500:1
• IDL < 10 fg (industry first)
• Improved thermal profile
• “Standby” post run
• “Ready to run” from standby
• “Bake Mode” as needed
• Factory backflush option
• Fast Vent capability
• DS42i rough pump“standby”
power reduction
Better Vacuum by
Agilent Pump
• New Agilent V304 Turbo
• TwisTorr
Faster Scanning
• 20,000 u/s
• But never equal to the 7200 Q-
• Molecular Drag technology
• Improved pumping light gases
• H2 (220 L/s); He (255 L/s)
• Agilent Floating Suspension
• Longer life
• Quiet operation
TOF speed or ‘scan’ sensitivity
Safety/Protection
• Vacuum failure  Shut off gas
(He or H2)
7890B GC “Direct
Communications
Features
• EMF, calculator, etc.
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Agilent Confidential
January 8, 2013
New Extractor Ion Source for 5977
Why the Extractor EI Source?
Increase in ion count
 True increase in sensitivity
Better trace level precision
 Lower IDL and MDL
Proven sensitivity increase demonstrated with
over 1500 7000 Series GC/TQ!
Page 13
Agilent Confidential
January 8, 2013
Comparison of Inert and Extractor Ion Source
Repeller
(Push)
Ionization area
Ions
Drawout Plate
Extractor Lens
(Pull)
Ion Focus Lens
Entrance Lens
Mass Filter
Inert Ion Source
Extractor Source
More ions to
Mass Filter
Agilent Confidential
Page 14
January 8, 2013
Extractor Source “Etune” Sensitivity Increase
Blue – Extractor 40ppb solution (SVOC)
Red – Standard 40ppb solution (SVOC)
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Agilent Confidential
January 6, 2013
More improvements on 5977’s Extractor Ion Source:
Improved Thermal Profile
Why is this important?
Better performance of high boilers
i.e. PAH even at lower concentration!
Good repeatability of
PAH even at the trace
level (5ppb)!!
10 Injections
80 min analysis
Improvement for better Mass
Spectrometer of Gas Chromatograph
Page 16
January 15, 2013
Comparing repeatability of 80min Method
5975 result
Two years ago
5977 result
5977 result
200ppb PAH
5ppb PAH
Phthalates analysis with 5977 GC/MSD
Page 17
Oct 2012
Key features of “Direct Communication” between
7890B and 5977A
Sleep/Wake mode
• Sleep mode saves power (320W  190W)
 Lower lab operating cost
Safety Control
• If 5977A MSD pump fail, 7890B will shut off the gas supply
 Helium carrier gas : gas waste is eliminated
 Hydrogen carrier gas: risk of explosion is reduced
Cool Down MSD Faster (FastVent)
• When MSD Vent Cycle started, GC will cool down MSD analyzer at the
optimum gas flow to MSD.
 40% faster when cooling down from 280C to 100C (< 25 min)
Page 18
Agilent Confidential
January 8, 2013
Saving Energy and Time
400
350
300
250
200
150
100
50
0
Power
saved
Time saved using Fast Vent
250
200
Source Temp
Power( VA)
Power Consumption 5977
150
100
Normal
Op
Standby
Fast
Vent
50
Vent
0
0
4
6
Time(min)
19
7
15
20
23
30
35
40
Time saved
Better HW Features: Agilent Turbo Pump (V304)
Innovation for:
•Higher pumping performance
• especially for He and H2
•High reliability
• lower cost of operation
Agilent Quality Standard
TwisTorr 304 FS
Press release : http://www.agilent.com/about/newsroom/presrel/2012/19dec-ca12077.html
Page 20
Agilent Confidential
January 8, 2013
TwisTorr 304 FS: Vacuum Design
8 EDM turbo stages
(rotor) designed to
optimize pumping
speed for light
gases
8 Turbo stators
made by pressed
SST sheet
technology
3 TwisTorr drag stages (spiral
channels) designed for high
compression ratio
performances
Agilent’s new patentpending Floating
Suspension for
robust, low noise
operation
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Better HW Features: Agilent Rough Pumps
New DS42i
High performance
Energy saving design
“Inverter” not compatible with diff pump
New IDP-3c
High performance
Oil-free scroll pump
NH3 CI compatible
with Agilent Quality Standards
Page 22
Agilent Confidential
January 8, 2013
Agilent Inert Flow Path … From Injection to Detection
…now from a single supplier
Ultra Inert Inlet Liner
New
Ultimetal Inlet Weldment, Shell
and Transfer Lines
Ultimetal – TCD,
FPD, NPD/FID Jets
Ultra Inert Gold Seal
Ultimetal Capillary Flow Technology
Devices, Ultimate Union
Ultra Inert GC Column
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Agilent Confidential
January 8, 2013
Programmable Helium Conservation Module
Works with Gas Saver function of 7890 based GC
Standby Method can be programmed after the sequence ends
Analytical methods are unchanged since Helium is still the carrier during the run
5977A GC/MSD IDL Specification
IDL is a statistically derived value from the precision (%RSD) of 8
consecutive, splitless injections of 100fg OFN at 99 % confidence
level. The more precise instruments (lower %RSD) will generate lower
IDLs.
The Agilent 7890B/5977A with an ALS or CTC autosampler has the
following specifications:
Ion Source type
High Vacuum pump type
IDL of OFN by 8 replicates of ALS*
Extractor Source
Turbo Pump
10fg
Turbo Pump
24fg
Diffusion Pump
30fg
Inert Source
These are installation specification, performed on customer site, immediately
after installation and carry a lot more value than a reference specs.
*Systems without an Autosampler will have only S/N specification.
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January 15, 2013
Why IDL specification is useful for the 5977 in SIM?
Influence of Noise in S/N specs
•
In SIM (or MS/MS) when the Noise is too low, such as during acquisition of
an OFN standard, S/N results may not give the best characterization
•
If the noise level is insignificant - varies a lot and too low- the derived value
from it (S/N) also becomes insignificant
Value of IDL
•
IDL is derived from reproducibility/precision results, the same metrics most
users utilize every day in their work. The IDL statistical process is the same
used for Method Detection Limit calculations, and the %RSD values are tools
to monitor analytical performance
•
IDL characterizes the whole system – AS, GC, MS- giving confidence in
overall reliability. It is the results of 8 consecutive injections, unlike S/N in a
single shut, which can be influenced by many parameters/conditions
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January 15, 2013
The Influence of the low Noise on S/N values
Same Data File, RMS, 5 Second Noise Region
A 0.01 min. shift in the noise region resulted a 6000 higher S/N value.
When the noise is insignificant (low), the derived S/N value is also insignificant!
Noise region: 5.65min - 5.73 min
S/N: 10785
S/N
Noise region:
5.65min-5.73 min
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Noise region: 5.66min - 5.74 min
S/N: 3335
S/N
Noise region:
5.66min-5.74 min
January 15, 2013
Market Leading Performance
Premium Value: The Best Just Got Better
Detector
Performance
MSD
Scan 1500:1 S/N; SIM 10 fg IDL – Best in Class with IDL
tested at installation when ALS or Combi available (with
Extractor Source)
FID
<1.4 pg C/sec
ECD
<4.4 fg/sec
FPD
<2.5 pg S/sec; <45 fg P/sec
SCD
<0.5 pg S/sec
NPD
Best for P, 0.08 pg/sec N beat only by Thermo 0.05 pg/sec–
but only using OUR Blos bead!
TCD
400 pg/mL
No one can now offer a better specification package than Agilent
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Agilent Confidential
January 8, 2013
Market’s Best S/N (SNR) > 1500:1
Market’s Best Scan Rate = 20,000 u/s
Market’s ONLY IDL < 10 fg
Better
from Agilent
Statistical confirmation of entire system’s
performance
Page 29
Agilent Confidential
January 8, 2013
Mass Hunter GC/MSD Software
MassHunter Qual
MH
MassHunter Quant
CS
MassHunter GC/MS Acquisition
MSD ChemStation DA
And Use Either
MassHunter Data Analysis
OR
MSD ChemStation Data Analysis
G1701FA
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Agilent Confidential
January 8, 2013
MSD ChemStation
Standard interface
Quantitation
Macros
QEdit
Reports
Library Searching
MSD ChemStation DA
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Agilent Confidential
January 8, 2013
MassHunter WorkStation Qualitative Analysis
Chromatogram
Results
Data
Navigator
Method
Explorer
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Method
Editor
Spectrum Results
Agilent Confidential
January 8, 2013
Mass Hunter Quantitative Analysis
The Quant Batch
screen looks like this.
 Results Information
for the batch can be
displayed. Visual guides
highlight specific data
that fall outside specific,
predefined conditions.
 Compound Information
displays graphical
representation of the
peak, qualifier
information, spectral
information, and the
ISTD. Visual guides
help identify associated
data problems.
Results
Information
Compound Information
Batch Information
(i.e., Calibration Curve)
 Batch Information
provides easy
visualization and
customization of
relevant desired data.
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Agilent Confidential
January 8, 2013
Unknowns Analysis
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Agilent Confidential
January 8, 2013
MassHunter Excel Reporting
•Excel Based (Excel 2010 supported)
•MassHunter application exports data in XML and graphics files
•Templates define report formatting
•Over 100 shipping report templates
•Reports can be altered for desired information & appearance
•Customization using Excel features (function, formatting, code)
•Computer Based Training for report customization by users
•Some customization available through Agilent
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Agilent Confidential
January 8, 2013
Application Notes
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Agilent Confidential
January 8, 2013
Flexibility: Performance and Budget
Balance of Price,
GC performance,
& MS performance
Lowest Price
5977A Turbo MS
Performance Meets
5977A Diff MS
Method Requirement
7890B GC
5977E Turbo MS
Inert source
5977E Diff MS
7820A
Stainless source
Page 37
Highest
GC Performance
& MS Performance
5977A EI
5977A EI/CI
7890B GC
Extractor source
DS42i
Maintenance and Support Issues
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Agilent Confidential
January 8, 2013
Q&A Discussion
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Agilent Confidential
January 8, 2013

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