Synergy H4 Applications

Report
Examples of Synergy H4 Applications:
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AlphaScreen/AlphaLISA
Apoptosis
Bacterial adhesion
BRET/BRET2
Cell adhesion
Cell expression
Cell proliferation
Cell viability & Cytotoxicity
Chemotaxis
Colorimetric assays
Cytokine analysis
DNA quantification
eGFP quantification
ELISA
Enzyme activity
Enzyme kinetics
FRET
Heterogeneous TRF (DELFIA)
Homogeneous TRF (LANCE)
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Immunoassays
Kinase activity
Metabolic activity
Mitochondrial membrane potential
NADH/NADPH
Na+/H+ exchange
Nucleic acid quantification
Oxidative burst
Oxidation reactions
PCR product quantification & quality
Protease activity
Protein quantification
Receptor binding studies
Reporter gene
Signal Transduction
Single Nucleotide Polymorphisms
Abs/FI spectral scanning
Toxicology
Examples of Absorbance/Photometric Applications:
Assay
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AlamarBlue®
Amplex® Red
phosphoELISA
Vybrant assay C12 Rezarurin
Vybrant MTT Cell Proliferation Assay Kit
Lipid Peroxidation Assay Kit (using MDA)
Neutral Red Uptake Assay
CellTiter 96® Cell Proliferation Assay (MTT)
DC Protein, Bradford, Lowry & BCA Protein Assays
OxiSelect™ Superoxide Dismutase Activity Assay
Pierce 660nm Protein Assay
Glycoprotein Carbohydrate Estimation Kit
Colorimetric Protease Assay Kit
Micro BCA Protein Assay Kit
FluoReporter Biotin-XX Protein Labelling Kit
Sulforhodamine B Assay
WST-1 Cell Proliferation Assay
Cell Counting Kit-8 (CCK-8)
IDEXX FlockChek™ Avian Influenza Multi-species ELISA Assay
Melamine ELISA Assay
CaspACE™ Assay
MTT Assay
Growth Assay (OD at 600nm)
Haemoglobin denaturation Assay
Target
Cell Proliferation, Viability & Counting
Enzyme Activity
Kinases
Metabolic Labeling
Cell Counting
Oxidative Stress
Cell Proliferation
Cell Proliferation
Protein Quantification
Oxidative Stress
Protein Quantification
Glycoprotein Quantification
Protease
Protein Quantification
Biotin
Cell Proliferation
Cell Counting
Cell Counting
Avian Influenza
Food Safety
Apoptosis
Cell Proliferation
Cell Growth
Haemoglobin
Examples of Fluorescence Intensity Applications:
Assay
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Adapta® Universal Kinase Assay
AlamarBlue®
Amplex® Red
CyQuant® Cell Proliferation Assay Kit
EnzCheck
Fluo-4 AM
GeneBLAzer GPCR
LIVE/DEAD® Viability/Cytotoxicity Kit
NDP Sensor Assay
Omnia® Kinase Assays
PiPer™ Phosphate Assay Kit
SYTOX® stains
Vivid® CYP450
Z'-LYTE™
Quant-iT™ PicoGreen® dsDNA Assay Kit
Transcreener® GDP FI Assay
Fura-2
Indo-1
FluxOR™ Potassium Ion Channel Assay Kits
OxiSelect™ ROS Assay Kit
CellTiter-Fluor™ Cell Viability Assay
BD™ Ratiometric Calcium Assay Kit
FluoroBlok Cell Invasion Assays (using calcein AM)
Target
Kinases
Cell Proliferation, Viability & Counting
Enzyme Activity
Cell Proliferation
Proteases
GPCR
GPCR
Apoptosis
Kinases
Kinases
Phosphatases
Viability
Cytochrome P450
Kinases
Nucleic Acid Quantification
GTPase
GPCR
GPCR
Ion Channels
Oxidative Stress
Cell Viability
GPCR
Cell Migration
Examples of Luminescence Applications:
Assay
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CellTiter-Glo® Luminescent Cell
Dual-Glo® Luciferase Assay
Dual-Luciferase® Reporter (DLR™) Assay
Bright-Glo™ Luciferase Assay
Steady-Glo® Luciferase Assay
ONE-Glo™ Luciferase Assay
P450-Glo™ Assay
ADP-Glo™ Assay
Caspase-Glo® 3/7 Assay
cAMP-Glo™ Assay
GloSensor™ cAMP Assay
Aequorin
Photina®
Britelite™ plus Reporter Gene Assay
Steadylite plus™ Reporter Gene Assay
Neolite Reporter Gene Assay
ATPlite™ Luminescence Assay
aCella -TOX™ Bioluminescence Cytotoxicity Assay
ToxiLight® Non-destructive Cytotoxicity BioAssay Kit
ATP quantification assay (luciferine-luciferase)
Target
Cell Proliferation
Gene Expression
Gene Expression
Gene Expression
Gene Expression
Gene Expression
CYP Activities
Kinase
Apoptosis
GPCR
GPCR
GPCR
GPCR
Gene Expression
Gene Expression
Gene Expression
Cell Proliferation/Kinases
Cell Death
Cell Death/Cell Proliferation
ATP Quantification
Examples of Time-Resolved Fluorescence Applications:
Assay
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LanthaScreen™ Kinase Assay
LanthaScreen™ Cofactor Recruitment Assays
LanthaScreen™ Cellular assay
Transcreener® ADP TR-FRET Assay
LANCE® cAMP Detection Kit
DELFIA® cAMP Kit
DELFIA® Tyrosine Kinase Kit
DELFIA® EuTDA Cell Cytotoxicity Kit
DELFIA® Cell Proliferation Kit
PI3-Kinase HTRF Assay
HTRF® HTplex™ cAMP and IP-One Assay
HTRF® cAMP femto 2 Kit
HTRF® KinEASE™ Kits
HTRF® heparanase Assay
HTRF® Ubiquitin Assays
Human Insulin TRF-Pincer™ Assay
Tag-lite® Receptor Ligand Binding Assay
Cellul'erk
HTScan® Kinase Assays
Target
Kinases
Nuclear Receptors
Kinases
Kinases
GPCR
GPCR
Kinases
Cytotoxicity
Cell Proliferation
Cell Proliferation
GPCR
GPCR
Kinases
Heparanases
Ubiquitinases
Insulin
GPCR
GPCR
Kinases
Examples of Fluorescence Polarization Applications:
Assay
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PolarScreen™
PolarScreen™ Competitive Binding Assays
Transcreener® AMP/GMP Assay
Transcreener® GDP FP Assay
Transcreener® UDP Assay
Transcreener® ADP2 FP Assay
[FP]2
Target
Kinases
Nuclear Receptors
PDE’s
GTPase
UDP-glycosyltransferases
Kinases
cAMP
Examples of AlphaScreen Applications:
Assay
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AlphaScreen® cAMP Assay
AlphaScreen® SureFire® Assay
AlphaLISA® Immunoassay Kits
Target
GPCR
GPCR
Biological Markers (see below)
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Biologics: IgA, IgG, IgM, CHO-P, MSO-P, E.Coli HCP, Residual Protein A
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Angiogenesis: TNFα, VEGF, VEGFB, VEGFC, VEGFD
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Cancer: AFP, CA125, CXCL11/I-TAC, EGF-R, EPO, ERBB2/HER2, IFNβ, MMP1, MMP2, MMP9, β-NGF, PSA
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Cardiovascular: Myeloperoxidase, NT-proBNP, PCSK9, Plasminogen, Renin/Prorenin, tPA, ICAM-1
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Neurodegeneration: Aβ 1-40, Aβ 1-42, sAPPα, sAPPβ, Aβ 1-15/16
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Metabolic: Adiponectin, GH, GLP-1, IGF1, IGF2, Insulin, Leptin, Prolactin, Albumin
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Virology: HIV p24
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Inflammation:
CCL2/MCP1, CCL3/MIP-1α, CCL4/MIP-1β, COMP, CRP, CXCL10/IP-10, G-CSF, IFNα, IFNγ,
IL1α, IL1β, IL2, IL3, IL4, IL5, IL6, IL7, IL8, IL10, IL12 (p70), IL13, IL17, IL18, CXCL1/GROα,
CXCL9/MIG, IL15
Enabling technologies covering the full spectrum of targets:
AlphaScreen
GPCR Functional Assays:
cAMP
IP3
GTP Binding
Receptor Binding Assays:
GPCRs
Nuclear
Enzyme Assays:
Tyrosine Kinase
Serine/Threonine Kinase
Phosphatase
Protease
Transferase
Polymerase
Phospholipase
Helicase
Phosphodiesterase
Protein Interactions:
Protein:Protein
Protein:Peptide
Protein:DNA
Other Functional:
Cell Proliferation/Viability
TRF
LANCE/HTRF
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